作为一位科学家,一生中最荣耀的时刻之一就是研究成果被世人认同。

张医师于2011年至美国纽约大学担任全职科学家,于2005年在国际期刊中发表了崭新学术论文。
“Developmental incompetency of denuded mouse oocytes undergoing“Developmental incompetency of denuded mouse oocytes undergoing
maturation in vitro is ooplasmic in nature and is associated with aberrant Oct-4 expression”

论文内容指出“早期学者发现小鼠卵子发育至GV时期,其受精及后续胚胎发育之生长因子皆以筹备完成,因为认为GV时期后的卵子不需要卵丘细胞的支持。”但在张医师研究中发现“oct-4”在胚胎发育中扮演重要角色。

GV时期至MII时期的卵子在发育过程若有卵丘细胞的支持,其oct-04在胚胎发育过程中是正常的;反之若GV时期至MII时期,卵子没有卵丘细胞支持,其oct-04表现是相当混乱的。若使用这些没有卵丘细胞支持的卵子进行受精后移植至母体体内并不能活产小鼠。

此篇论文发表著名期刊 [Human Reproduction]中,世界著名生殖医药学专家 “Ryuzo Yanagimachi”也因为张医师所发表的论文而改变他原本的想法观念并寄明信片致谢。

近年这篇论文更被论文期刊中的权威 [ Nature cell biology]引用,
引用张医师文章的论文是“Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes”

在这篇论文的参考资料中,张医师的论文排在第六名,
同时张医师以前在纽约大学的同事 James A Grifo,寄信道贺张医师研究被世人所认同。

这篇崭新论文除了被期刊中的权威 [ Nature cell biology]引用外,还被许多期刊及教科书收录其中,世界各地学者一致认同张医师的研究。

以下是引用此篇论文的期刊与教科书。

题目 第一作者 作者国籍 投稿期刊 impact factor
自然受精及体细胞移置兔胚之基因表现与发育潜能探讨 陈建宏 台湾 台大生物科技研究所学位论文 硕士论文
单层细胞共培养对猪去卵丘卵母细胞体外成熟和孤雌发育的影响 顾晓龙 中国 《中国兽医学报》2011年 第4期 604-608页 0.661
山羊卵泡大小对卵母细胞体外发育能力的影响 吴志南 中國 《苏州大学学报:医学版》2009年 第5期 891-893页 共3页 已停刊
Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche Stephany El-Hayek 加拿大 Molecular Mechanisms of Cell Differentiation in Gonad Development pp 191-224 已列入
教科书
The Use of Micromanipulation Methods as a Tool to Prevention of Transmission of Mutated Mitochondrial DNA HelenaFulka 捷克 Current Topics in Developmental Biology Volume 77, 2007, Pages 187-211 4.000
A Method for Chronological Intravital Imaging of Bovine Oocytes during In Vitro Maturation Morten R. Petersen 丹麥 Microscopy and Microanalysis 14, 549–560, 2008 1.891
Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification Yang Yu 中国 Human Reproduction, Vol.24, No.3 pp. 649–657, 2009 5.020
OCT-4 Gene Expression Pattern In Different Grades Of Buffalo (Bubalus bubalis) Oocytes Ch. Srinivasa Prasad 印度 Buffalo Bulletin (June 2010) Vol.29 No.2 0.104
Co-culture embedded in cumulus clumps promotes maturation of denuded oocytes and reconstructs gap junctions between oocytes and cumulus cells Guixue Feng 中国 Zygote 21 (August), pp. 231–237. 1.053
Coculturing denuded oocytes during the in vitro maturation of bovine cumulus oocyte complexes exerts a synergistic effect on embryo development S.R. Dey 韩国 Theriogenology 77 (2012) 1064–1077 1.986
Concanavalin-A Induces Granulosa Cell Death and Inhibits FSH-Mediated Follicular Growth and Ovarian Maturation in Female Rats Ethel V. Velasquez 智利 Endocrinology,  2013, 154(5):1885–1896 4.286
Cyclic AMP elevating agents promote cumulus cell survival and hyaluronan-matrix stability thereby prolonging the time of mouse oocyte fertilizability Monica Di Giacomo 义大利 The Journal of Biological Chemistry, 291 (2016), pp. 3821-3836 4.125
Developmental Potential of Selectively Enucleated Immature Mouse Oocytes Upon Nuclear Transfer A.A. MOHAMMED 波兰 MOLECULAR REPRODUCTION AND DEVELOPMENT 75:1269–1280 (2008) 2.316
Effects of cumulus cells on rabbit oocyte in vitro maturation Y. Tao 中国 Journal of Animal Physiology and Animal Nutrition 92 (2008) 438–447 1.244
Effects of Ooplasm Manipulation on DNA Methylation and Growth of Progeny in Mice Yong Cheng 中国 BIOLOGY OF REPRODUCTION 80, 464–472 (2009) 3.432
EVALUATION OF CONTRACEPTIVE PROPERTIES OF CILOSTAZOL (A PHOSPHODIESTERASE 3A INHIBITOR) IN MICE Glen Laine 美国 Biomedical Sciences 已列入

教科书

Effect of maturation medium on in vitro cleavage of canine oocytes fertilized with fresh and cooled homologous semen B. de A´ vila Rodrigues 巴西 Zygote 15 (February), pp. 43–53. 1.053
Injection of Mammalian Metaphase II Oocytes with Short Interfering RNAs to Dissect Meiotic and Early Mitotic Events Manami Amanai 日本 BIOLOGY OF REPRODUCTION 75, 891–898 (2006) 3.432
Intrinsic quality of goat oocytes already found denuded at collection for in vitro embryo production Joanna Maria Gonçalves Souza-Fabjan 法国 Theriogenology Nov;86(8 )2016 1–10 1.986
Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice Xiaoqian Wang 澳洲 Reproduction (2009) 138 527–535 3.100
Maturation and Developmental Competence of Selectively Enucleated Germinal Vesicle Oocytes of Mammals upon nuclear transfer Abd El-Nasser Ahmed Mohammed 英国 International Journal of Current Engineering and Technology, Vol.4, No.1 (Feb 2014) 1.274
Mechanisms contributing to the reduced developmental
competence of glucosamine-exposed mouse oocytes
Cheryl J. Schelbach 澳洲 Reproduction, Fertility and Development, 2010, 22, 771–779 2.656
Morphometric assessment of in vitro matured dromedary camel oocytes determines the developmental competence after parthenogenetic activation Islam M. Saadeldin 埃及 Theriogenology 1.986
Mouse Cumulus-Denuded Oocytes Restore Developmental Capacity Completely When Matured with Optimal Supplementation of Cysteamine, Cystine, and Cumulus Cells Ping Zhou 中国 BIOLOGY OF REPRODUCTION 82, 759–768 (2010) 3.432
Paracrine factors from cumulus-enclosed oocytes ensure the successful maturation and fertilization in vitro of denuded oocytes in the cat model Natasha M. Godard, M.Sc. 美国 Fertility and Sterility Vol. 91, No. 5, Supplement, May 2009 4.373
Putrescine supplementation during in vitro maturation
of aged mouse oocytes improves the quality of blastocysts
Dandan Liu 中国 Reproduction, Fertility and Development 2.656
Recovery of Functional Oocytes from Cultured Premeiotic Germ Cells After Kidney Capsule Transplantation Bo Chen 中国 STEM CELLS AND DEVELOPMENT Volume 22, Number 4, 2013 3.562
Somatic cells regulate maternal mRNA translation and
developmental competence of mouse oocytes
Jing Chen 美国 NATURE CELL BIOLOGY VOLUME 15 NUMBER 12  DECEMBER 2013 20.06
Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos Chien-Hong Chen 台湾 Reproductive BioMedicine Online (2012) 24, 433– 442 3.249
The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice Cheng-Jie Zhou 中国  Peer J. 2016;4:e1761. 2.177
The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts Y. H. Choi 美国 Reproduction (2009) 138 589–599 3.100
無卵丘水牛卵母细胞体外成熟方法的初步探討 冯貴雪 中国 畜牧兽医学报 2007, 38(6):614~619 0.593

在美国短短四年时间,就发表了震惊医学界的研究,张医师就是您所要寻找的医师。

该论文亦收录於「百度学术」中,与中国生殖医学领域教学相长。