做為一位科學家,一身中最榮耀的時刻之一就是研究成果被世人認同做為一位科學家,一身中最榮耀的時刻之一就是研究成果被世人認同
張醫師於2001年至美國紐約大學擔任全職科學家,於2005年在國際期刊中發表了嶄新的學術論文“Developmental incompetency of denuded mouse oocytes undergoing maturation in vitro is ooplasmic in nature and is associated with aberrant Oct-4 expression”
論文內容指出 “ 早期學者發現小鼠卵子發育至GV 時期,其受精及後續胚胎發育之生長因子皆已籌備完成,因此認為GV時期後的卵子不需要卵丘細胞的支持。” 但在在張醫師的研究中發現” oct-4 “在胚胎發育中扮演重要腳色。GV時期至MII時期的卵子在發育過程若有卵丘細胞的支持,其oct-4的表現在胚胎發育的過程中是正常的;反之若GV時期至MII時期,卵子沒有卵丘細胞支持,其oct-4的表現是相當混亂的。若使用這些沒有卵丘細胞支持的卵子進行受精後移植至母體體內並不能活產小鼠。
此篇論文發表致著名期刊 [Human Reproduction]中,世界著名生殖醫學專家 “Ryuzo Yanagimachi” 也因為張醫師所發表的這篇論文而改變他原本的想法及觀念並寄明信片致謝
近年這篇論文更被論文期刊中的權威 [ nature cell biology] 引用,引用張醫師文章的論文是 “Somatic cells regulate maternal mRNA translation and developmental competence of mouse oocytes” 。在這篇文章的參考資料中張醫師的論文是排在第六的位置。同時張醫師以前在紐約大學的同事 James A Grifo,寄信道賀張醫師的研究被世人認同。
這篇嶄新的論文除了被期刊中的權威 [ nature cell biology] 引用外還被許多期刊及教科書收錄,世界各地的學者一致認同張醫師的研究。

 

題目 第一作者 作者國籍 投稿期刊 impact factor
Somatic cells regulate maternal mRNA translation and
developmental competence of mouse oocytes
Jing Chen 美國 NATURE CELL BIOLOGY VOLUME 15 NUMBER 12  DECEMBER 2013 20.06
自然受精及體細胞核移置兔胚之基因表現與發育潛能探討 陳建宏 台灣 台大生物科技研究所學位論文 碩士論文
单层细胞共培养对猪去卵丘卵母细胞体外成熟和孤雌发育的影响 顾晓龙 中國 《中国兽医学报》2011年 第4期 604-608页 0.661
山羊卵泡大小对卵母细胞体外发育能力的影响 吴志南 中國 《苏州大学学报:医学版》2009年 第5期 891-893页 共3页 已停刊
Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche Stephany El-Hayek 加拿大 Molecular Mechanisms of Cell Differentiation in Gonad Development pp 191-224 已列入
教科書
The Use of Micromanipulation Methods as a Tool to Prevention of Transmission of Mutated Mitochondrial DNA HelenaFulka 捷克 Current Topics in Developmental Biology Volume 77, 2007, Pages 187-211 4.000
A Method for Chronological Intravital Imaging of Bovine Oocytes during In Vitro Maturation Morten R. Petersen 丹麥 Microscopy and Microanalysis 14, 549–560, 2008 1.891
Assessment of the developmental competence of human somatic cell nuclear transfer embryos by oocyte morphology classification Yang Yu 中國 Human Reproduction, Vol.24, No.3 pp. 649–657, 2009 5.020
OCT-4 Gene Expression Pattern In Different Grades Of Buffalo (Bubalus bubalis) Oocytes Ch. Srinivasa Prasad 印度 Buffalo Bulletin (June 2010) Vol.29 No.2 0.104
Co-culture embedded in cumulus clumps promotes maturation of denuded oocytes and reconstructs gap junctions between oocytes and cumulus cells Guixue Feng 中國 Zygote 21 (August), pp. 231–237. 1.053
Coculturing denuded oocytes during the in vitro maturation of bovine cumulus oocyte complexes exerts a synergistic effect on embryo development S.R. Dey 韓國 Theriogenology 77 (2012) 1064–1077 1.986
Concanavalin-A Induces Granulosa Cell Death and Inhibits FSH-Mediated Follicular Growth and Ovarian Maturation in Female Rats Ethel V. Velasquez 智利 Endocrinology,  2013, 154(5):1885–1896 4.286
Cyclic AMP elevating agents promote cumulus cell survival and hyaluronan-matrix stability thereby prolonging the time of mouse oocyte fertilizability Monica Di Giacomo 義大利 The Journal of Biological Chemistry, 291 (2016), pp. 3821-3836 4.125
Developmental Potential of Selectively Enucleated Immature Mouse Oocytes Upon Nuclear Transfer A.A. MOHAMMED 波蘭 MOLECULAR REPRODUCTION AND DEVELOPMENT 75:1269–1280 (2008) 2.316
Effects of cumulus cells on rabbit oocyte in vitro maturation Y. Tao 中國 Journal of Animal Physiology and Animal Nutrition 92 (2008) 438–447 1.244
Effects of Ooplasm Manipulation on DNA Methylation and Growth of Progeny in Mice Yong Cheng 中國 BIOLOGY OF REPRODUCTION 80, 464–472 (2009) 3.432
EVALUATION OF CONTRACEPTIVE PROPERTIES OF CILOSTAZOL (A PHOSPHODIESTERASE 3A INHIBITOR) IN MICE Glen Laine 美國 Biomedical Sciences 已列入
教科書
Effect of maturation medium on in vitro cleavage of canine oocytes fertilized with fresh and cooled homologous semen B. de A´ vila Rodrigues 巴西 Zygote 15 (February), pp. 43–53. 1.053
Injection of Mammalian Metaphase II Oocytes with Short Interfering RNAs to Dissect Meiotic and Early Mitotic Events Manami Amanai 日本 BIOLOGY OF REPRODUCTION 75, 891–898 (2006) 3.471
Intrinsic quality of goat oocytes already found denuded at collection for in vitro embryo production Joanna Maria Gonçalves Souza-Fabjan 法國 Theriogenology Nov;86(8 )2016 1–10 1.838
Live offspring from vitrified blastocysts derived from fresh and cryopreserved ovarian tissue grafts of adult mice Xiaoqian Wang 澳洲 Reproduction (2009) 138 527–535 3.184
Maturation and Developmental Competence of Selectively Enucleated Germinal Vesicle Oocytes of Mammals upon nuclear transfer Abd El-Nasser Ahmed Mohammed 英國 International Journal of Current Engineering and Technology, Vol.4, No.1 (Feb 2014) 1.274
Mechanisms contributing to the reduced developmental
competence of glucosamine-exposed mouse oocytes
Cheryl J. Schelbach 澳洲 Reproduction, Fertility and Development, 2010, 22, 771–779 2.135
Morphometric assessment of in vitro matured dromedary camel oocytes determines the developmental competence after parthenogenetic activation Islam M. Saadeldin 埃及 Theriogenology 1.838
Mouse Cumulus-Denuded Oocytes Restore Developmental Capacity Completely When Matured with Optimal Supplementation of Cysteamine, Cystine, and Cumulus Cells Ping Zhou 中國 BIOLOGY OF REPRODUCTION 82, 759–768 (2010) 3.471
Paracrine factors from cumulus-enclosed oocytes ensure the successful maturation and fertilization in vitro of denuded oocytes in the cat model Natasha M. Godard, M.Sc. 美國 Fertility and Sterility Vol. 91, No. 5, Supplement, May 2009 4.426
Putrescine supplementation during in vitro maturation
of aged mouse oocytes improves the quality of blastocysts
Dandan Liu 中國 Reproduction, Fertility and Development 2.656
Recovery of Functional Oocytes from Cultured Premeiotic Germ Cells After Kidney Capsule Transplantation Bo Chen 中國 STEM CELLS AND DEVELOPMENT Volume 22, Number 4, 2013 3.562
Spatial and temporal distribution of Oct-4 and acetylated H4K5 in rabbit embryos Chien-Hong Chen 台灣 Reproductive BioMedicine Online (2012) 24, 433– 442 3.249
The beneficial effects of cumulus cells and oocyte-cumulus cell gap junctions depends on oocyte maturation and fertilization methods in mice Cheng-Jie Zhou 中國  Peer J. 2016;4:e1761. 2.177
The uterine environment modulates trophectodermal POU5F1 levels in equine blastocysts Y. H. Choi 美國 Reproduction (2009) 138 589–599 3.100
無卵丘水牛卵母細胞體外成熟方法的初步探討 冯貴雪 中國 畜牧獸醫學報 2007, 38(6):614~619 0.593

在美國短短四年的時間,就發表了震驚醫學界的研究,張醫師就是你/妳所以找尋的醫師